Applied Technologies and Innovations

  Previous Article | Back to Volume | Next Article
  Abstract | References | Citation | Download | Preview | Statistics
Volume 2
Issue 2
Online publication date 2010-08-10
Title Experimental investigations of submerged fermentation and synthesis of pectinolytic enzymes by Aspergillus Niger : Effect of inoculum size and age of spores
Author Kiro Mojsov
Abstract The paper investigates effects of the different concentration of inoculums and age of spores on the synthesis of pectinolytic enzymes with the aim of their optimizing for maximal enzyme production and growth of the microorganism. The microorganism used in this work was the fungus Aspergillus niger MK-15, which was isolated from soil as a highly active producer of pectinolytic enzymes. The results showed that the concentration of the inoculum and age of spores have a pronounced effect on the pectinolytic activity and dry weight. Obtained results suggest that for submerged fermentation and synthesis of pectinolytic enzymes by Aspergillus niger MK-15, the optimal inoculum concentration is 3% or 6x106 spores and optimal age of spores is 3 days old.
Acunã-Arguelles, M., Gutiérrez-Rojas, M., Viniegrea-González, G.,  Favela-Torres, E., 1995. “Production  and  properties of three pectinolytic activities produced by Aspergillus niger  in submerged and solid-state fermentation,” Appl. Microbiol. Biotechnol,  Vol.43, pp.808-814    

Aguilar, G. and Huitron, C. , 1986. “Application of fed-batch cultures in the production of extracellular pectinases by Aspergillus sp.,” Enzyme and Microbial Technology, Vol. 9, pp.541-45

Bailey, M. and Pessa, E., 1990. “Strain and process for production of polygalacturonase,” Enzyme and   Microbial Technology, Vol.12, pp.266-71

Berovič, M. and Ostroveršnik H., 1997. “Production of  Aspergillus  niger   pectolytic  enzymes   by  solid state bioprocessing of apple pomace,” Journal of Biotechnology, Vol.53, pp.47-53 

Friedrich, J., Cimerman, A., Steiner, W., 1989. “Submerged production of pectolytic  enzymes by Aspergillus niger : effect of different aeration /agitation regimes”, Applied Microbiology and Biotechnology, Vol. 31, pp.490-94

Kuhad, R., Manchanda, M., Singh, A., 1998. “Optimization of xylanase production by a hyperxylanolitic mutant strain of Fusarium oxysporum,” Process Biochem, Vol.33, pp.641-47

Larios, G., García, J. and  Huítrón, C., 1989. “Endo-polygalacturonase production from  untreated lemon peel by Aspergillus sp. CH-Y-1043,” Biotechnology Letters, Vol.11, pp.729-34

Leuchtenberger, A., Friese, E., Ruttloff, H., 1989. “Variation of polygalacturonase and pectinesterase synthesis by aggregated mycelium of Aspergillus niger in dependence on the source,” Biotechnology Letters, Vol.11, pp.255-58

Maria Lucia Garcia Simoes and Samia Marta Tauk-Tornisielo, 2006. “Optimization of xylanase biosynthesis by Aspergillus japonicas isolated from a “Caatinga” area in the Brazilian state of Bahia,” African Journal of Biotechnology, Vol.5(11), pp.1135-141

Naidu, G., Panda, T., 1998. “Production of pectolytic enzymes - a review,” Bioprocess Engineering, Vol.19,  pp.355-61

Peričin, D., Jarak, M., Antov, M., Vujičič, B., Kevrešan, S., 1992. “Effect of inorganic phosphate on the secretion of pectinolytic enzymes by Aspergillus niger,” Letters in Applied Microbiology, Vol.14, pp. 275-78

Qinnghe, C., Xiaoyu, Y., Tiangui, N., Cheng, J., Qiugang, M., 2004. “The screening of culture condition and properties of xylanase by white-rot fungus Pleurotus ostreatus,” Process Biochem., Vol.39, pp.561-66

Schmidth, O., Angermann, H., Frommhold-Treu, I., Hoppe, K., 1995. “Experimental and theorical nvestigation  of  submerged  fermentation and synthesis of pectinolytic  enzymes by Aspergillus niger”,  Appl. Microbiol. Biotechnol, Vol.43, pp.424-30

Shah, A.R., Madamwar, D., 2005. “Xylanase production by a newly isolated Aspergillus foetidus strain and its characterization”, Process Biochem., Vol. 40, pp. 1763-771

Sikyta, B., 1983. “Development of microbial process”, Method. Ind. Microb., pp.250-74

Smith, D., Wood, T., 1991. “Xylanase production by Aspergillus awamorri development of a medium and optimization of extracellular xylanase while maintaining low protease production,” Biotechnol. Bioeng., Vol.38, pp.883-90

Keywords Pectinolytic enzymes, apple pulp,inoculum size, age of spores, Aspergillus niger, optimization
Pages 40-45
Download Full PDF Download
  Previous Article | Back to Volume | Next Article
Search in articles
Journal Published articles
ATI 263
Journal Hits
ATI 799604
Journal Downloads
ATI 7737
Total users online -