Applied Technologies and Innovations

  Previous Article | Back to Volume | Next Article
  Abstract | References | Citation | Download | Preview | Statistics
Volume 7
Issue 2
Online publication date 2012-06-21
Title Trichoderma harzianum as a biocontrol agent against Alternaria alternata on tobacco
Author Biljana Gveroska, Jugoslav Ziberoski
Trichoderma fungi are the most popular agents used in a biological control. Therefore, our aim was to determine an impact of Trichoderma harzianum on the fungus Alternaria alternata - a causing agent of the brown spot disease on tobacco. In vitro analyses were made in several variants of double culture, in order to study the effect of difusible and volatile metabolites. 
There was strong reducing effect on the development of A.alternata with various mechanisms of antagonistic influence. The volatile metabolites have also shown reducing effect. Some abnormalities were observed in the pathogen's morphology both in difusible and volatile metabolites.  The strong reducing effect of T.harzianum  towards A. alternata can be applied in biological control of this pathogen.

Almeida, F., Cerqueira, F., Silva, R., Uhoa, C., Lima, A., 2007. “Mycoparatism studies of Trichoderma harzianum strains against Rhizoctonia solani: Evaluation of coiling and hydrolytic enzyme production,” Biotechnol Lett., Vol.29(8), pp.1189-193

Barbosa, M., Rehm, K., Menezes, M., Mariano, R.L., 2001. “Antagonism of Trichoderma species on Cladosporium herbarum and their enzimatic characterization,” Brazilian Journal of Microbiology, Vol.32, pp.98-104

Gang, T., Xing Zhong, L., Wang, G., Shidong, L., 2004. “Role of chitinase by Trichoderma sp. in biocontol of tobacco brown spot caused by Alternaria alternate,” Chinese Journal of Biological Control, Vol.20(4), pp.252-55 
Ghildyal, A., Pandey, A.,  2008. “Isolation of cold tolerant antifungal strains of Trichoderma sp. from glacial sites of Indian Nimalayan Region,” Research Journal of Microbiology, Vol.3(8), pp.559-64

Harman, G.E., 1996. “Trichoderma for biocontrol of plant pathogens: From basic research to comercialized products,” Conference on Biological Control, Cornell community, April 11-13

Harman, G.E., 2006. “Overview of mechanisms and uses of Trichoderma spp.,” Phytopathology, Vol.96, pp.190-94

Izzati, N., Abdulah, F.,  2008. “Disease suppression in Ganoderma-infected oil palm seedlings treated with Trichoderma harzianum,”  Plant Protect.Sci., Vol.44(3), pp.101-107

Küçük, Ç., Kivanç, M., 2003. “Isolation of Trichoderma Spp. and determination of their antifungal, biochemical and physiological fetures“, Turk J Biol., Vol.27, pp.247-53

Lieckfeldt, E., Samuels, G., Nirenberg, H., Petrini, O., 1999. “A morphological and molecular perspective of Trichoderma viride: Is it one or two species,” Appl Environ Microbiol., Vol.65(6), pp.2418-428 

Mandare, V., Suryawanshi, A., Gawade, S.,  2008. “Studies on Alternaria blight of chickpea,” Agricultural Science Digest, Vol.28(3), pp.222-24
Mirkova, E., 1982. “Isledvanje in vitro na antagonistickata aktivnost na Trichoderma sp. km nekoi pocveni patogeni,” Gradinarska i lozarska nauka, god. XIX, No.1, pp.73-81 

Mirkova, E., 1983. “Prilozenie na Trichoderma harzianum Rifai za borba s fuzariinoto uvrhvane (Fusarium oxysporum sch.f. dianthi (Prill. Et Del.) Snyd. Et Hans.) pri oranzerinia karamfil,” Selskostopanska akademia, Gradinarska i lozarska nauka, god. XX, No.1, pp.65-69 

Monte, E., 2001. “Understanding Trichoderma: Between biotechnology and microbial ecology,” Int. Microbiol., Vol.4, pp.1-4

Ranasingh, N., Saturabh, A., Nedunchezhiyan, M., 2006. “Use of Trichoderma in disease managemant,” Orissa Review, September-October, pp.68-70

Roco, A., Pérez, L., 2001. “In vitro biocontrol activity of Trichoderma harzianum on A.alternata in the presence of growth regulators,” Plant Biotechnology, Vol.4(2), pp.68-73

Rosado, I., Rey, M., Codon, A., Gonavites, J., Moreno-Mateos, M.A., Benitez, T., 2007. “QID74 Cell wall protein of Trichoderma harzianum is involved in cell protection and adherence to hydrophobic surfaces,” Fungal Genetics and Biology, Vol.44(10), pp.950-64

Sempere, F., Santamarina, M., 2007. “In vitro biocontrol analysis of Alternaria  alternata(Fr.) Keissler under different environmental conditions,” Mycopathologia, Vol.163(3), pp.183-90

Ubalua, A., Oti, E., 2007. “Antagonistic properties of Trichoderma viride on post harvest cassava root rot pathogens,” African Journal of Biotechnology, Vol.6,(21), pp.2447-450

Umamaheswari, B., Thakore, B., More, T., 2009. “Post-harvest management of ber (Ziziphus mauritiana Lamk) fruit rot (Alternaria  alternata (Fr.) Keissler) using Trichoderma species, fungicides and their combinations,” Crop Protection, Vol.28(6), pp.525-32

Keywords Pathogen, antagonist, inhibitory effect, diffusible/volatile metabolites, abnormalities
Pages 67-76
Download Full PDF Download
  Previous Article | Back to Volume | Next Article
Search in articles
Journal Published articles
ATI 263
Journal Hits
ATI 755748
Journal Downloads
ATI 7631
Total users online -