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Volume 12
Issue 3
Online publication date 2012-07-27
Title Identification and antibacterial activity of methanol extract of Luffa acutangula Roxb.
Author Resmi Mustarichie, Zalinar Udin, Muchtaridi , Supriyatna
Abstract Antibacterial activity of Luffa acutangula Roxb. (angled luffa) has been assayed against some pathogenic bacterial. Fruit powder of angled luffa was macerated with methanol, and the methanol extract extracted sequentially with hexane, chloroform, ethyl acetate and buthanol. Antibacterial activity was evaluated by well diffusion method. Extract with the highest antibacterial activity was identified regarding their class of compounds using phytochemical screening and Thin Layer Chromatography (TLC) method. The antibacterial activity of the extract was compared with that of the ampicillin used. The methanol extract inhibited the growth of the P. aeruginosa, E. Coli, B. Subtilis and S.aureus, but did not inhibit the growth of the E. Aerogenes, S. dysentriae and S. thypi. The ethyl acetate extract showed the highest antibacterial activity against P. aeruginosa, E. Coli, B. Subtilis and S. aureus, followed by chloroform, buthanol, and hexane extract, respectively. The ethyl acetate extract possed phenolic, condensed tannin, flavonoids, saponins and terpenoids. Based on the MIC and the equivalent value of ethyl acetate extract compared with that ampicillin used, the antibacterial activity of ethyl acetate extract was lower than with that of the ampicillin used.
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References
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Keywords Luffa acutangula Roxb., antibacterial activity, well diffusion, methanol extract, ethyl acetate extract
Pages 70-77
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