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Volume 14
Issue 1
Online publication date 2013-04-25
Title Effectiveness of xenotransplantation of human fetal hepatocytes in spleen of rats with acute liver failure induced by CCL4
Author Abdukhakim Khadjibaev, Maisa Urazmetova, Aybek Madaminov, Ravshan Mavlyan-Hodjaev, Raisa Ahmedova, Feruza Yusupova
Abstract Human’s fetal hepatocytes (HFH) were intrasplenic transplanted white non-pedigree rats with acute  liver failure (ALF) challenged by single per oral administration of hepatotropic toxin diluted in oil ССl4 at a dose 10 ml/kg (volumetric correlation 1:1) (10 mL/kg body weight as a 1:1 mixture of CCl4 and mineral oil). Transplantation had positive effect on all biochemical blood parameters of the studying animals. Morphologic study showed that reparative-restorative processes were arising in hepatic parenchyma after administration of HFH into splenic pulp of rats with model of ALF on days 14-21. Substantial and main factor in restoration of parenchyma was restoration of micro topographic interrelations in acinus as well as polyploidy of hepatic cells expressed in increase of hepatocytes’ nuclei sizes and hypertrophy of cells themselves. It is an indirect confirmation of engraftment of HFH in liver of rats with model of ALF.
Aripov, U., Pak, N., Islamov, B. et al., 1998. “Developing  an effective method of  perfusion   and conversion donor  liver  transplant   in   the  experiment,”   Medical   Journal   of   Uzbekistan   [Medicinskiy zhurnal Uzbekistana], in Russian, No.2, pp.89-91

Cheredeev, A., Gorlina, N., Kozlov, I., 1999. “CD-markers  in the practice of clinical diagnostic  laboratories,” Clin Lab Diagnostics [Klinich. lab. Diagnostika], in Russian, No.6, pp.25-32

Khajibayev, А.,  Urazmetova,  M., Madaminov, A.  et al.,  2011.  “Comparative    estimation  of    effect    of allogen  and  xenogeny  isolated  hepatocytes on immune state of  rats  with acute liver failure,” Bulletin of Emergency Medicine [Vestnik ekstrennoy mediciny], in Russian,  No.2, pp.48-51 

Laleman, W.,  Verbeke, L,   Meersseman, P. et al., 2011.  “Acute-on-chronic  liver  failure:  Current  concepts on definition, pathogenesis, clinical manifestations and potential therapeutic interventions,” Expert Rev Gastroenterol Hepatol, Vol.4(5), pp.523-37

Lu, B., Gralla, J., Liu, E. et al., 2008. “Evaluation of a scoring system  for  assessing  prognosis  in pediatric acute liver failure,” Clin Gastroenterol Hepatol, Vol.10(6), pp.1140-145

Onishenko, N., Klimenko, E., Pozdnyakov, O. et al., 2006. “Cellular  therapy  as  method  of  correction of pathogenetic disorders  in  dyslipidemia  and  early  stages  of  atherogenesis,” Bulletin  of  the  Russian  Academy of medical sciences [Vestnik Rossiyskoy Akademii  medicinskih  nauk], in  Russian,  No.9-10, pp.88-95

Runovich, А.,  Pivovarov, Yu.,  Kurilskaya, T. et al., 2005.  “Changes of  indices  of  lipid  metabolism  and hemostasis system in intravenous and  intrahepatic  transplantation of neonatal  allogen  hepatocytes in  conditions of experimental hypercholesterolemia,” Pathophys. & Exper. Ther. [Patologicheskaya   fiziologiya i eksperimentalnaya terapiya], in Russian, Vol.1., pp.14-16

Sakaida, I.,  Terai, S.,  Yamamoto, N.  et al., 2004.  “Transplantation  of  bone  marrow  cells  reduces  CCl4-induced liver fibrosis in mice,” Hepatology, Vol.40(6), pp.1304-311

Shimanko, I., Musselius, S., 1993. Acute liver failure [Ostraya pechenochno-pochechnaya  nedostatochnost], Moscow

Strekalovskiy, D., Nikiforov, S., Goldberg, O. et al., 1998. “ Effect of transplantation  of  fetal  liver tissue on the  morphological  changes  in  experimental  atherosclerosis”,  Bulletin  of  Experimental  Biology and Medicine, [Vestnik Eksperementalnoy biologii i mesdicini], in Russian, Appl.1, pp.134-37

Teng, Y., Wang, Y., Li, S. et al., 2010. “Treatment  of  acute  hepatic  failure  in  mice  by transplantation of mixed microencapsulation of rat hepatocytes and transgenic human fetal liver stromal cells”, Tissue Eng Part C Methods, Vol.16(5), pp.1125-134

Touboul, T., Vallier, L., Weber, A., 2010. “Robust differentiation of fetal hepatocytes from human embryonic  stem cells and iPS,” Med Sci (Paris), Vol.26(12), pp.1061-1066

Weibel, E., Kistler, G., Scherie, W., 1966.  “Practical  stereological  methods for morphometric cytology,” J. Cell Biol., Vol.30, pp.23-28

Zalyalieva, M.,  2004.  Methods   for  determination  of   lymphocyte  subpopulations  in  humans  [Metody  ocenki subpopulyaciy limfocitov perifericheskoy krovi u cheloveka], in Russian, Tashkent

Zhang, L., Han, F., Wu, D. et al., 2010. “Analysis  of  the  clinical features of and  responsive factors on the prognosis  in  patients  with  fulminant  hepatic  failure,”  Zhonghua  Gan  Zang Bing Za Zhi, Vol.18(8), pp.614-17

Zhou, P., Lessa, N., Estrada, D. et al., 2011. “Decellularized liver matrix as a carrier for the transplantation of human fetal and primary hepatocytes in mice,” Liver Transpl, Vol.17(4), pp.418-27

Keywords Human’s fetal hepatocytes, acute liver failure, carbon tetrachloride poisoning, intrasplenic transplantation hepatocytes, morphology
Pages 52-60
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