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Volume 17
Issue 1
Online publication date 2016-07-15
Title Development of a new antiviral medication for the grippe
Author Valentina Divocha
Abstract
The grippe till now is a mass disease resulted in hospitalization of thousands of sick persons and high mortality rate. The objective of the work presented is to extract inhibitor of trypsin - like proteinases from the waste the first stage of the industrial process of gamma globulin preparation from donated human blood, and to study its protective properties at the flu under experiment. Derivation the pure inhibitor included the following stages: the enzyme excretion, ultrasonic disintegration of cells, ion-change chromatography on DEAE-cellulose, dialysis, lyophilic drying. The method described allows obtaining five isoforms with inhibitory activity. The greatest amount of trypsin-like proteinases was registered in the isoform of the 5th fraction. The 5th isoform with high inhibitory activity was used for the study of therapeutically properties and the experimental grippe A in white mice. The isoform mentioned showed a promoted protective effect.
Citation
References
Mitamura, K. and Sugaya, N., 2006. "Diagnosis and Treatment of influenza-clinical investigation on viral shedding in children with influenza", Viruses, Vol.56, pp.109-116

Veremeyenko, K.N, 1994. "Proteolytic enzymes and their inhibitors, new applications in the clinic", Vrachebnoye Delo, pp.8-13. (in Russian)

Veremeyenko, K.N., Goloborodko, O.P., Kizim A.I., 1988. Proteolysis in normal and pathological conditions, Kiev: Health (in Russian)

Divocha, V.A., 2010. Patent Ukraine № 89778. Method trypsin protease inhibitor allocation of waste reception gamma globulin albumin and blood person / applicant and patentee; publ. 10.03.2010, Bull. N 5.

Hendon, Y.I., 2009.  Problems prevention influenza in pregnant women and newborn children, Vopr. Vyrusol 4 (2009) 4-10. (in Russian)

Hendon, Y.I., Markushin, S.G., Anopova, I.I. et al., 2005 "Further development of the culture (MDCK) live cold-adapted influenza vaccine: The vaccine strains in the cultivation of industrial enzymes", Problems Virology, Vol.2, pp.4-9. (in Russian)

Divocha V.P., Mykhalchuk, V.M., Gozhenko, A.I., 2006a. Patent number 21599 Ukraine, IPC (2006), A 61 K 36/00. The method of selection of protease inhibitor trypsin of waste reception gamma globulin and albumin human blood № u 2006 11233; appl. 25.10.2006; publ. 15.03.2007, Bull. N 3. (in Russian)

Divocha V.P., Mykhalchuk, V.M., Gozhenko, A.I., 2006b. Availability trypsin protease and its inhibitor in the waste receiving gamma globulin, Medical Chemistry 8 (2006) 60-63. (in Russian)

Divocha V.P, Mikelashvyly, M.T., 2001. The presence of protease inhibitors trypsin donor blood in humans and in waste handling sera production of natural science at the turn stolety: Internat. Conf., Sochi, 8-10 October 2001. Abstracts. Moscow, 3 (2001) 63. (in Russian)

Keywords Inhibitors, proteinases, virus grippe, chromatography
DOI http://dx.doi.org/10.15208/mhsj.2016.02
Pages 9-15
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