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Volume 8
Issue 4
Online publication date 2011-07-29
Title Ofloxacin analysis validation method in human blood plasma (in vitro) using solid-phase extraction HPLC
Author Resmi Mustarichie, Wiwiek Indriyati, Iyan Sopyan
Abstract Until now, analysis of Ofloxacin in human blood plasma using solid-phase extraction (SPE) by HPLC UV detector has not been reported. This study aims to determine the validity of analytical methods in Ofloxacin study in human blood plasma (in vitro) using an HPLC SPE UV detector. Plasma samples were extracted by SPE. Analytes were analyzed using a C18 column (octadecylsilane) 250x4.6 mm, particle size 10 μm, mobile phase 85,5:14,5 v v 0.025 M phosphate buffer (pH 2.2) and acetonitrile with a flow rate of 2 ml/min, detection performed at 294 nm with the internal standard ciprofloxacin. Validated analytical method was based on the parameters: selectivity, accuracy, precision, repeatability, linearity, LOD, LOQ, and the suitability of the system. Validation analysis showed selectivity test Rs>1.5, test repeatability with CV(%) <10%, linearity was obtained in the range of 0.1 to 6 µg/ml with correlation coefficient (r) from 0.9998 to 0.9999. Based on the area ratio of peak height and a segment of the chromatogram obtained LOD values 0.023 and 0.024 µg/ml, LOQ value of 0.076 and 0.080 tg/ml, percent accuracy from 94.32 to 100.45% and 97.68 to 101.63%, and precision CV (%) 0.31 to 0.85% and 0.84 to 1.08%. System suitability test results on the retention time, area ratio, and high ratios of peak chromatogram shows the CV(%) <10%. Can be concluded that the analytical methods used have validity in accordance with the requirements. 
Baruah, H., Roy, D., Roy, R., and Khonikor, H., 2004. “Pharmacokinetics, tissue residue and plasma protein binding of ofloxacin in goats,” J. Vet Sci. 5(2), pp.97-101

Caufield, W., and Stewart, J., 2002. “Determination of zidovudin and levofloxacin in human plasma by reversed phase HPLC and solid phase extraction,” J. Liq. Chrom. & Related, Tech., Vol.25(12), pp.1791-805

Esposito, S., Noviello, S., D’Errico, G., Motta, G., Passali, D., Aimoni, C., Pilucchi, S., Fallani, M., Cassetta, I., Mazzei, T., and Novelli A., 2006. “Concentration of moxifloxacin in plasma and tonsillar tissue after multiple administration in adult patients. J. Antimicr. Chemother., Vol.57, pp.789-792

Ganiswarna, S., Setiabudy, R., Suyatna, F., 1995. “Purwantyastuti, dan Nafrialdi,” Farmakologi dan Terapi., Edisi IV. Jakarta: Gaya Baru

Harmita., 2006. “Analisis Fisikokimia”. Buku Ajar, Jakarta, Departemen Farmasi FMIPA UI
Indrayanto, G., 1994. “Metoda validasi pada analisis dengan kromatografi,” Medika-Jurnal Kedokteran & Farmasi, Vol.20(2), pp.49-51

Lai, C., Lee, T., Au, K., and Chan, A., 1997. “Uniform solid-phase extraction procedure for toxicological drug screening in serum and urine by HPLC with photodiode-array detection,” Clin. Chem., Vol.43(2), pp.312-25

Nepali, N., Alam, K., Subish, P., and Khan, G., 2007. “Hemoglobinurea due to ofloxacin in a 9 year old child,” Pharmacologyonline, Vol.1, pp.1-5

Rehak, V., 2004. “Determination of ofloxacin in serum and blood”. Available at: http://www.galleryscience. com [access on 26 June 2009]

Rose, M., Bygrave, J. and Stubbings, G., 1998. “Extension of multi-residue methodology to include the determination of quinolones in food,” Analyst. 123, pp.2789-796
Snyder, L., Kirkland, J. and Glajch. J., 1997. “Practical HPLC Method Development,” 2nd Edition, New York, John Willey & Sons, Inc.

Sultana, N., Arayne, M. and Yasmeen, N., 2007. “In vitro availability of ofloxacin in presence of metals essential to human body”, J. Pharm. Sci., Vol.20(1), pp.36-42

Tanwar, Y., Patel, D. dan Sisodia, S., 2007. “In vitro and in vivo evaluation of ocular inserts of ofloxacin,” DARU, Vol. 15(3), p.139

Wacke, R., Forster, S., Adam, U., Mundkowski, R.,. Klar, E., Hopt, U. and Drewelow, B., 2006. “Penetration of moxifloxacin into the human pancreas following a single intravenous or oral dose”, J. Antimicr. Chemother., Vol.58(5), pp.994-9

Keywords HPLC, ofloxacin, SPE, human plasma
Pages 80-87
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