Medical and Health Science Journal

  Previous Article | Back to Volume | Next Article
  Abstract | References | Citation | Download | Preview | Statistics
Volume 9
Issue 5
Online publication date 2011-11-05
Title Activity of leaf extracts of Coix lachryma Linn. and Asparagus cochinchinenesis Linn. as breast anticancer drugs
Author Resmi Mustarichie, Zalinar Udin, Jutti Levita, Ida Musfiroh, Ikal Zulfricar
Abstract In the current economic crisis, the use of plant medicine for cancer prevention should be investigated. Coix lachryma Linn and Asparagus cochinchinenesis Linn are among eleven  of species of medicinal plants that are noted as plant medicine for cancer in Indonesia, although their mechanism of action are still unknown.  The eleven plants were screened using in vitro methods, Sulforhodamin B against breast cancer cells (MCF-7) and skin (KB). The research included a maceration process using ethanol as solvent and an anti-cancer testing process in vitro using Sulforhodamin B indicated by the value of percentage viability. Extracts were classed as being 'active anticancer' if they showed IC50 values below 100 ppm.. Coix lachryma Linn. and Asparagus cochinchinenesis Linn. show breast and skin anticancer activity with IC50 values 6.51 ppm and 11.3 ppm of MCF-7 cells. The ethanol plant extracts were further extracted using various solvents with increasing polarity: n-hexane, methylene chloride, and ethyl acetate. The methylene chloride extract of  Coix lachryma Linn. had IC50 = 2.75 ppm against MCF-7 cells. Against KB cells, methylene chloride extracts of Coix lachryma  Linn. gave IC50 = 5.16 ppm. For Asparagus cochinchinenesis Linn., an ethyl acetate extract had IC50 = 3.70 ppm against KB cancer cells and IC50 = 9.80 ppm against MCF-7 cancer cells. These data indicated that both plants can be used as anticancer drugs on breast and skin cancers.
Anonymous, 1983. Pemanfaatan Tanaman Obat, Edisi III, Direktorat Jendral Pengawasan Obat dan Makanan, Jakarta

Atta-ur-Rahman and Choudary, M., 1998. New trends in natural product chemistry, Harwood Academic Publishers, Switzerland

Colegate, S. and Molyneux, R., 1993. Bioactive natural products, detection, isolation and structural determination, CRC Press., Inc., United States of America

Dalimarta, S., 1999. Ramuan Tradisional untuk Pengobatan Kanker,  Penebar Swadaya, Jakarta
Dalimarta, S., 2003.  Atlas Tumbuhan Obat Indonesia, Cetakan I, Trubus Agriwidya, Jakarta
Endo, A. 1987. “Drugs inhibiting HMG-CoA reductase,” International encyclopedia of pharmacology and therapeutics, Vol.31, pp.257-67

Farnsworth, N., 1966. “Biological and phytochemical screening of plants,” J. Pharmaceutical Sci., Vol.55(3), 137-41

Hariana, A., 2006. Tanaman Terpilih untuk Pengobatan Berbagai Jenis Kanker, Seri Agrisehat, Tumbuhan Obat dan Khasiatnya, Seri 1, 2, dan3, Penebar Swadaya

Hutapea, J., 1993. Inventarisasi Tanaman Obat Indonesia, Departemen Kesehatan R.I.

Kinghorn, A., 1985, “A phytochemical approach to bioscreening of natural products,” in Thompson, E. (Ed.), Drug Bioscreening, Fundamentals of Drug Evaluation Techniques in Pharmacology, Graceway Publishing Co., New York, pp.113-31

Likhitwitayawuid, K., Angerhofer, C., Chai, H., Pezzuto, J., Cordell, G., 1993. “Cytotoxic and antimalarial alkaloids from the tubers of Stephania pierrei,” J. Nat. Prod., Vol.56, pp.1468-478

Skehan, P., Storeng, R., Scudiero, D., Monks, A. et al., 1990. “New colorimetric cytotoxicity assay for anticancer-drug screening,” J. Nat., Vol.82(13), pp.1107-112

Uripi, V., 2002. Menu untuk Penderita Kanker, Puspa Swara, Jakarta

Keywords Ethanol extract, breast anticancer, Coix lachryma Linn, Asparagus cochinchinenesis Linn, IC50, Sulforhodamin B, MCF-7
Pages 47-57
Download Full PDF Download
  Previous Article | Back to Volume | Next Article
Search in articles
Journal Published articles
MHSJ 940
Journal Hits
MHSJ 2562110
Journal Downloads
MHSJ 17477
Total users online -