Medical Microbiology and Immunology

  Previous Article | Back to Volume | Next Article
  Abstract | References | Citation | Download | Preview | Statistics
Sample volume 3
Title Regional spread of HIV-1 M subtype B in middle-aged patients by random env-C2V4 region sequencing
Author Martin Sturmer, Katrin Zimmermann, Carlos Fritzsche, Emil Reisinger, Gottfried Doelken
Abstract A transmission cluster of HIV-1 M:B was identified in 11 patients with a median age of 52 (range 26–65) in North-East Germany by C2V4 region sequencing of the env gene of HIV-1, who—except of one—were not aware of any risky behaviour. The 10 male and 1 female patients deteriorated immunologically, according to their information made available, within 4 years after a putative HIV acquisition. Nucleic acid sequence analysis showed a R5 virus in all patients and in 7 of 11 a crown motif of the V3 loop, GPGSALFTT, which is found rarely. Analysis of formation of this cluster showed that there is still a huge discrepancy between awareness and behaviour regarding HIV transmission in middle-aged patients, and that a local outbreak can be detected by nucleic acid analysis of the hypervariable env region.
Citation
References
1. Gottlieb MD, Schroff R, Schanker HM, Weissman JD, Fan PT,
Wolf RA, Saxon A (1981) Pneumocystis carinii pneumonia and
mucosal candidiasis in previously healthy homosexual men. N
Engl J Med 305:1425–1431
2. Hamouda O, Marcus U, Voss I, Kollan C (2007) Epidemiology of
HIV infection in Germany. Bundesgesundbl Gesundforsch Gesundschutz 50:399–411
3. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with
chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–
5467
4. Van de Peer Y, De Wachter R (1997) Construction of evolutionary distance trees with Treecon for Windows: accounting for
variation in nucleotide substitution rate among sites. Comput
Appl Biosci 13:227–230
5. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular
evolutionary genetics analysis (MEGA) software version 4.0.
Mol Biol Evol 24:1596–1599
6. Briggs DR, Tuttle DL, Sleasman JW, Goodenow MM (2000)
Envelope V3 amino acid sequence predicts HIV-1 phenotype (coreceptor usage and tropism for macrophages). AIDS 14:2937–
2939
7. Balfe P, Simmonds P, Ludlam CA, Bishop JO, Brown AJ (1990)
Concurrent evolution of human immunodeficiency virus type 1 in
patients, infected from the same source: rate of sequence change
and low frequency of inactivating mutations. J Virol 64:6221–
6233
8. Ji J, Loeb LA (1994) Fidelity of HIV-1 reverse transcriptase
copying a hypervariable region of the HIV-1 env gene. Virology
199:323–330
9. Salazar-Gonzales JF, Bailes E, Pham KT, Salazar MG, Guffey
MB, Keele BF, Derdeyn CA, Farmer P, Hunter E, Allen S,
Manigart O, Mulenga J, Anderson JA, Swanstrom R, Haynes BF,
Athreya GS, Korber BTM, Sharp PM, Shaw GM, Hahn BH
(2008) Deciphering human immunodeficiency virus type 1transmission and early envelope diversification by single-genome
amplification and sequencing. J Virol 82:3952–3970
10. Leitner T, Kumar S, Albert J (1997) Tempo and mode of
nucleotide substitutions in gag and env gene fragments in human
immunodeficiency virus type 1 populations with a known transmission history. J Virol 71:4761–4770
11. Diaz RS, Zhang L, Busch MP, Mosely JP, Mayer A (1997)
Divergence of HIV-1 quasispecies in an epidemiologic cluster.
AIDS 11:415–422
12. Bele´c L, Si MA, Muller-Trutwin MC, Gilquin J, Gutmann L,
Safar M, Barre´-Sinoussi F, Kazatchkine MD (1998) Genetically
related human immunodeficiency virus type 1 in three adults of a
family with no identified risk factor for intrafamilial transmission.
J Virol 72:5831–5839
13. Kane CT, Montavon C, Toure MA, Faye MA, Ndlaye AG, Diallo
AG, Ndoye I, Liegeois F, Delaporte E, Mboup S, Peeters M
(2001) Full-length genome sequencing of HIV-1 type 1 group O
viruses isolated from a heterosexual transmission cluster in
Senegal. AIDS Res Human Retroviruses 17:1211–1216
14. Lemey P, van Dooren S, van Laethem K, Schrooten Y, Derdelinckx I, Goubau P, Brun-Ve´zinet F, Vaira D, Vandamme AM
(2005) Molecular testing of multiple HIV transmissions in a
criminal case. AIDS 19:1649–1658
15. Buskin SE, Ellis GM, Pepper GG, Frenkel LM, Pergam SA,
Gottlieb GS, Horwitch C, Olliffe JF, Johnson K, Shallit P, Heinen
C, Schwartz M, Wood RW (2008) Transmission cluster of multiclass highly drug-resistant HIV-1 among 9 men who have sex
with men in Seattle/King County, WA, 2005-2007. J Acquir
Immune Defic Syndr 49:205–211
16. Clumeck N, Taelman H, Hermans P, Piot P, Schoumacher M, De
Wit S (1989) A cluster of HIV infection among heterosexual
people without apparent risk factors. N Engl J Med 321:1460–
1462
17. Robbins KE, Weidle PJ, Brown TM, Saekhou AM, Coles B,
Holmberg SD, Folks TM, Kalish ML (2002) Molecular analysis
in support of an investigation of a cluster of HIV-1 infected
women. AIDS Res Human Retroviruses 18:1157–1161
18. Sagar M, Laeyendecker O, Lee S, Gamiel J, Wawer MJ, Gray
RH, Serwadda D, Sewankambo NK, Shepherd JC, Toma J,
Huang W, Quinn TC (2008) Selection of HIV variants with
signature genotypic characteristics during heterosexual transmission. J Infect Dis 199:580–589
19. Brenner BG, Roger M, Routy JP, Moisi D, Ntemgwa M, Matte C,
Baril JG, Thomas R, Rouleau D, Bruneau J, Leblanc R, Legault
M, Tremblay C, Charest H, Wainberg M (2007) High rates of
forward transmission events after acute/early HIV-1 infection. J
Infect Dis 195:951–958
20. Taylor M, Rotblatt H, Brooks JT, Montoya J, Aynalem G, Smith
L, Kenney K, Laubacher L, Bustamante T, Kim-Farley R,
Fielding J, Bernrard B, Daar E, Kerndt R (2007) Epidemiologic
investigation of a cluster of workplace HIV infections in the adult
film industry: Los Angeles, California 2004. Clin Infect Dis
44:301–305
21. Leitner T, Escanilla D, Franzen C, Uhlen M, Albert J (1996)
Accurate reconstruction of a known HIV transmission history by
phylogenetic tree analysis. Proc Natl Acad Sci USA 93:10864–
10869
22. Kaye M, Chibo D, Birch C (2009) Comparison of Bayesian and
maximum-likelihood phylogenetic approaches in two legal cases
involving accusations of transmission of HIV. AIDS Res Human
Retroviruses 25:741–748
23. Leitner T, Foley B, Hahn B, Marx P, McCutchan F, Mellors J,
Wolinsky S, Korber B (2007) HIV Sequence Compendium 2006/
2007. Los Alamos National Laboratory, Los Alamos
24. Van der Bij AK, Dukers NH, Couthino RA, Fennema HS (2008)
Low HIV testing rates and awareness of HIV infection among
high-risk heterosexual STI clinic attendees in The Netherlands.
Eur J Public Health 18:376–379
25. Boerma JT, Stansfield SK (2007) Health statistics 1. Health statistics now: are we making the right investments. Lancet
369:779–786
26. Coates TJ, Richter L, Caceres C (2008) Behavioural strategies to
reduce HIV transmission: how to make them work better. Lancet
372:669–684
27. Knapper CM, Roderick J, Smith J, Temple M, Birley HD (2008)
Investigation of an HIV transmission cluster centred in South
Wales. Sex Transm Infect 84:377–380
28. Mitchell CM, Kaufman CE, Beals J (2004) Identifying diverse
HIV risk groups among American Indian young adults: the utility
of cluster analysis. AIDS Behav 8:263–275
29. Ndumbe P, Atchou G, Biwole M, Lobe V, Ayuk-Takem J (1993)
Infections among pygmies in the Eastern province of Cameroon.
Med Microbiol Immunol 182:281–284
30. Wicker S, Rabenau HF, Gottschalk R, Doerr HW, Allwinn R
(2007) Seroprevalence of vaccine preventable and blood transmissible viral infections (measles, mumps, rubella, polio, HBV,
HCV and HIV) in medical students. Med Microbiol Immunol
196:145–150
31. Stu¨rmer M, Doerr HW, Gu¨rtler L (2009) Human immunodefi-
ciency virus: 25 years of diagnostic and therapeutic strategies and
their impact on hepatitis B and C virus. Med Microbiol Immunol
198:147–155
Keywords HIV transmission - Epidemiology - Cluster - Risky behaviour - Local outbreak - Middle-aged people
Download Full PDF Download
  Previous Article | Back to Volume | Next Article
Share
Search in articles
Statistics
Journal Published articles
MMI 51
Journal Hits
MMI 123326
Journal Downloads
MMI 585
Total users online -